RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin β5 axis

Recently, long noncoding RNAs (lncRNAs) have been reported to play important role in the pathogenesis of various cancers. However, functions RNF185-AS1 hepatocellular carcinoma (HCC) remain unknown. The expression HCC cells and tissues was measured by quantitative real-time polymerase chain reaction (qRT-PCR). effects on tumor cell proliferation, migration invasion were assessed Cell Counting Kit-8 (CCK8) assay, colony formation transwell assay. luciferase reporter RNA-binding protein immunoprecipitation qRT-PCR Western blot performed explore confirm interaction between miR-221-5p integrin β5. progression explored through vivo experiments. highly expressed lines. High levels correlated with advanced TNM stage, distant metastasis a poorer overall survival rate. knockdown inhibited invasion. Additionally, acted as sponge for β5 identified target gene miR-221-5p. Rescue assays showed that inhibitor or overexpression rescued function migration, Moreover, we found growth metastasis. Our study demonstrates is new oncogenic lncRNA suggests RNF185-AS1/miR-221-5p/integrin axis might be potential therapeutic HCC.